Source identification of airborne Escherichia coli of swine house surroundings using ERIC-PCR and REP-PCR

Enterobacterial repetitive intergenic consensus (ERIC) sequences in Escherichia coli: Evolution and implications for ERIC-PCR.

Enterobacterial repetitive intergenic consensus (ERIC) sequences are 127-bp imperfect palindromes that occur in multiple copies in the genomes of enteric bacteria and vibrios. Here we investigate the distribution of these elements in the complete genome sequences of nine Escherichia coli (including Shigella species) strains. There is a significant tendency for copies to be adjacent to more high...

Assessment of Intra-Serotypic Diversity in Escherichia coli from Dogs Using ERIC-PCR

The scope of this study was to characterize the intra-serotypic genetic diversity in fecal Escherichia coli isolates obtained from dogs, using enterobacterial repetitive intergenic consensus (ERIC)-PCR. Serological typing of forty-seven E. coli isolates recovered from the rectal swabs (n=93) of different dog breeds in Andhra Pradesh revealed O141 (5 isolates), O128 (3 isolates), O126 (2 isolate...

Identification of Biofilm Encoding Genes (agg) in the Escherichia coli Isolates by Multiplex-PCR Method

Introduction: Biofilm is a collection of microbial cells that are irreversibly suppressed and mildly washed away. The aggR gene is located on the main plasmid (pAA), which codes for many actuator factors. The pathogen strains of EAEC have an aggR gene, the gene has been identified in six classes of pathogenic E. coli.   Materials & Methods: In the present study, 60 stool samples from children ...

Curli Production and ERIC-PCR Fingerprinting of Escherichia coli from Clinical Cases of Bovine Mastitis

Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.

A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli s...